板有效膜面(mian)積PHsF2直(zhi)徑mm)膜面(mian)積(cm2)產品類型iirool6-we1l站立式(shi)(shi)(shi)培(pei)(pei)(pei)養皿(min)4.23024-well站立式(shi)(shi)(shi)培(pei)(pei)(pei)養皿(min)0.6126-we1l懸(xuan)掛式(shi)(shi)(shi)培(pei)(pei)(pei)養皿(min)4.52412-well懸(xuan)掛式(shi)(shi)(shi)培(pei)(pei)(pei)養皿(min)1.11224-well懸(xuan)掛式(shi)(shi)(shi)培(pei)(pei)(pei)養皿(min)0.36.524-we11培(pei)(pei)(pei)養板0.71096-we1l培(pei)(pei)(pei)養板0.114六．電壓(ya)測量(liang)（膜電壓(ya)量(liang)程:士200.0 mV，靈敏度(du):0.1mV)1.將細胞置于室溫下平衡30分鐘(zhong);2.如(ru)前所述，測試Millicell ERS-2系統(tong)，并確保(bao)電極經(jing)過平衡;3.將模式(shi)(shi)(shi)調到(dao)Millivolts，開關打到(dao)ON;4.將電極模擬量(liang)輸(shu)出毫伏(fu)模式(shi)(shi)(shi)：1 mV = 10 mV歐姆模式(shi)(shi)(shi)：1Q = 1mV環境(jing)范圍(wei)50-100嚇F（10-38。C）默(mo)克細胞跨(kua)膜電阻(zu)儀保(bao)證質量(liang)-益啟生物公司。青浦區用于藥物ADME包括吸收分配等方面(mian)研(yan)究電阻(zu)儀

元的(de)(de)設計(ji)可以(yi)補償任何不對(dui)稱的(de)(de)測(ce)(ce)(ce)量(liang)。為(wei)了(le)進行電(dian)(dian)(dian)壓測(ce)(ce)(ce)量(liang)，需要(yao)將(jiang)(jiang)STX01電(dian)(dian)(dian)極(ji)(ji)(ji)平衡(heng)為(wei)使用(yong)前消(xiao)除任何偏移。為(wei)了(le)平衡(heng)，準備一(yi)種(zhong)與您將(jiang)(jiang)要(yao)使用(yong)的(de)(de)電(dian)(dian)(dian)解(jie)液(ye)類似(si)的(de)(de)電(dian)(dian)(dian)解(jie)液(ye)進行測(ce)(ce)(ce)量(liang)時(shi)，或使用(yong)磷酸鹽(yan)緩(huan)沖液(ye)（PBS），0.15M氯化鈉（NaCl）或0.1 M氯化鉀(jia)（KCI）。1.從(cong)充電(dian)(dian)(dian)器上(shang)斷(duan)開(kai)Millicell @ ERS-2電(dian)(dian)(dian)表(biao)。2.通(tong)過將(jiang)(jiang)插頭插入末端(duan)將(jiang)(jiang)STXO1電(dian)(dian)(dian)極(ji)(ji)(ji)連接到儀(yi)表(biao)將(jiang)(jiang)柔性(xing)電(dian)(dian)(dian)極(ji)(ji)(ji)電(dian)(dian)(dian)纜(lan)插入儀(yi)表(biao)的(de)(de)INPUT端(duan)口(kou)。3.關(guan)閉POWER開(kai)關(guan)，將(jiang)(jiang)MODE開(kai)關(guan)置于Ohms位(wei)置，將(jiang)(jiang)電(dian)(dian)(dian)極(ji)(ji)(ji)浸入電(dian)(dian)(dian)解(jie)液(ye)中。電(dian)(dian)(dian)極(ji)(ji)(ji)對(dui)通(tong)過儀(yi)器連接到儀(yi)器關(guan)閉電(dian)(dian)(dian)源，將(jiang)(jiang)它們在(zai)(zai)內(nei)部短接在(zai)(zai)一(yi)起，這樣(yang)可以(yi)使探黃浦區測(ce)(ce)(ce)量(liang)單層培養的(de)(de)細胞(bao)(bao)片的(de)(de)完整性(xing)電(dian)(dian)(dian)阻(zu)儀(yi)聯系方式(shi)上(shang)海益啟細胞(bao)(bao)跨膜電(dian)(dian)(dian)阻(zu)儀(yi)用(yong)于在(zai)(zai)細胞(bao)(bao)模型上(shang)進行內(nei)皮細胞(bao)(bao)通(tong)透性(xing)實(shi)驗。

意：兩次測量之間(jian)需要(yao)進行漂洗，以(yi)防止樣品進入殘(can)留物，請(qing)使(shi)用(yong)培養基，而不要(yao)使(shi)用(yong)蒸餾水。在(zai)(zai)(zai)電(dian)(dian)壓模式下，如果(guo)電(dian)(dian)表(biao)(biao)(biao)讀數為正，則表(biao)(biao)(biao)示(shi)基側細胞組(zu)織（粘(zhan)附于(yu)(yu)培養插入濾(lv)器的一側）的陽性相對于(yu)(yu)頂(ding)側（暴露）。相反，如果(guo)儀(yi)(yi)表(biao)(biao)(biao)正在(zai)(zai)(zai)讀取(qu)陰性，意味著基側相對于(yu)(yu)頂(ding)側為負。 維護和存儲系統 儀(yi)(yi)表(biao)(biao)(biao) 每月或在(zai)(zai)(zai)數字顯示(shi)屏上出(chu)現(xian)BATT時(shi)(shi)，請(qing)給電(dian)(dian)池(chi)(chi)充電(dian)(dian)。 在(zai)(zai)(zai)不使(shi)用(yong)儀(yi)(yi)表(biao)(biao)(biao)時(shi)(shi)，應(ying)關(guan)(guan)閉(bi)儀(yi)(yi)表(biao)(biao)(biao)電(dian)(dian)源并斷開(kai)與(yu)儀(yi)(yi)表(biao)(biao)(biao)的連接。 充電(dian)(dian)器，因為連續充電(dian)(dian)可能會縮短(duan)電(dian)(dian)池(chi)(chi)壽命。 注意：給電(dian)(dian)池(chi)(chi)充電(dian)(dian)時(shi)(shi)，請(qing)務必關(guan)(guan)閉(bi)儀(yi)(yi)表(biao)(biao)(biao)電(dian)(dian)源。 儀(yi)(yi)表(biao)(biao)(biao)應(ying)保持約8個小時(shi)(shi)的電(dian)(dian)量。它將關(guan)(guan)閉(bi) 電(dian)(dian)池(chi)(chi)電(dian)(dian)量低時(shi)(shi)自(zi)動(dong)避免損壞儀(yi)(yi)器。 當電(dian)(dian)池(chi)(chi)電(dian)(dian)量低時(shi)(shi)，

時(shi)不(bu)(bu)(bu)穩(wen)(wen)定充(chong)電(dian)(dian)(dian)(dian)(dian)(dian)器(qi)(qi)電(dian)(dian)(dian)(dian)(dian)(dian)源打(da)開電(dian)(dian)(dian)(dian)(dian)(dian)池電(dian)(dian)(dian)(dian)(dian)(dian)量低給電(dian)(dian)(dian)(dian)(dian)(dian)池充(chong)電(dian)(dian)(dian)(dian)(dian)(dian)12小(xiao)時(shi)，然(ran)后執(zhi)行“儀(yi)表(biao)功能檢查(cha)”。未(wei)能達成(cheng)低電(dian)(dian)(dian)(dian)(dian)(dian)量給電(dian)(dian)(dian)(dian)(dian)(dian)池充(chong)電(dian)(dian)(dian)(dian)(dian)(dian)12小(xiao)時(shi)，然(ran)后重復指定的(de)測(ce)試值“儀(yi)表(biao)功能檢查(cha)”。如(ru)果讀數在“米(mi)仍未(wei)指定，請(qing)與技術人員聯系(xi)功能性質服務。查(cha)看”間(jian)歇計(ji)電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)松(song)動或折斷更換電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)。讀連接(jie)器(qi)(qi)或電(dian)(dian)(dian)(dian)(dian)(dian)纜系(xi)統故障致電(dian)(dian)(dian)(dian)(dian)(dian)技術服務。不(bu)(bu)(bu)穩(wen)(wen)定的(de)電(dian)(dian)(dian)(dian)(dian)(dian)阻儀(yi)表(biao)已連接(jie)到(dao)斷開儀(yi)表(biao)與充(chong)電(dian)(dian)(dian)(dian)(dian)(dian)器(qi)(qi)的(de)連接(jie)。或電(dian)(dian)(dian)(dian)(dian)(dian)壓讀數，充(chong)電(dian)(dian)(dian)(dian)(dian)(dian)器(qi)(qi)（電(dian)(dian)(dian)(dian)(dian)(dian)壓漂移）電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)未(wei)浸入確(que)保(bao)兩個技巧都浸入溶(rong)(rong)液中(zhong)(zhong)。使用期間(jian)在文化媒體解決方案中(zhong)(zhong)注意：當儀(yi)表(biao)處(chu)于電(dian)(dian)(dian)(dian)(dian)(dian)壓模式(shi)時(shi)，它會在以(yi)下(xia)情況(kuang)下(xia)顯示器(qi)(qi)不(bu)(bu)(bu)穩(wen)(wen)定是正(zheng)常現象電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)未(wei)浸入溶(rong)(rong)液中(zhong)(zhong)。 糾正(zheng)措施不(bu)(bu)(bu)穩(wen)(wen)定的(de)阻力(li)電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)位置不(bu)(bu)(bu)對將電(dian)(dian)(dian)(dian)(dian)(dian)極(ji)(ji)(ji)(ji)上海益(yi)啟細胞(bao)跨(kua)膜電(dian)(dian)(dian)(dian)(dian)(dian)阻儀(yi)用于監控細胞(bao)單(dan)層的(de)致密程(cheng)度。

測定;細胞(bao)上(shang)的(de)零靜電(dian)(dian)(dian)(dian)荷消除了(le)DC電(dian)(dian)(dian)(dian)流對(dui)細胞(bao)膜(mo)產生的(de)不(bu)利影響(xiang);沒有金屬電(dian)(dian)(dian)(dian)極的(de)電(dian)(dian)(dian)(dian)化學沉積問(wen)題。MillicellERS-2細胞(bao)電(dian)(dian)(dian)(dian)阻儀(yi)（目錄號MERSO0002)的(de)規格參數與(yu)產品構成:膜(mo)電(dian)(dian)(dian)(dian)壓范(fan)(fan)圍(wei):土200.0 mv電(dian)(dian)(dian)(dian)壓測量(liang)(liang): 0.1 mv電(dian)(dian)(dian)(dian)阻范(fan)(fan)圍(wei): 0 ~ o999 o電(dian)(dian)(dian)(dian)阻分(fen)辨率:1Q交流方(fang)波電(dian)(dian)(dian)(dian)流:土10 uA nominal at 12.5 Hz電(dian)(dian)(dian)(dian)源:內置6V NiMH 2,200 mAH電(dian)(dian)(dian)(dian)池(chi)(chi)(chi)，外(wai)接12 VDC電(dian)(dian)(dian)(dian)源供充(chong)電(dian)(dian)(dian)(dian)每充(chong)電(dian)(dian)(dian)(dian)12h電(dian)(dian)(dian)(dian)池(chi)(chi)(chi)可(ke)用(yong)時(shi)間(jian):8-10 hrs模擬輸出:1-10 v (1 mV/ohm)環境(jing)要求:50-100 °F(10重量(liang)(liang)3磅（1.4公(gong)斤）當電(dian)(dian)(dian)(dian)池(chi)(chi)(chi)電(dian)(dian)(dian)(dian)量(liang)(liang)低于(yu)比較低閾值時(shi)，儀(yi)表自動關(guan)閉電(dian)(dian)(dian)(dian)源。上(shang)海益(yi)啟細胞(bao)跨膜(mo)電(dian)(dian)(dian)(dian)阻儀(yi)用(yong)于(yu)在細胞(bao)模型上(shang)進(jin)行藥(yao)物滲透實驗。青(qing)浦(pu)區用(yong)于(yu)藥(yao)物ADME包括吸收分(fen)配等方(fang)面研究(jiu)電(dian)(dian)(dian)(dian)阻儀(yi)

上海益啟生(sheng)物的細胞跨膜電阻儀詢價聯系方式。青浦區用于藥物ADME包括(kuo)吸收分配等(deng)方面研究電阻儀

泄和(he)毒性等(deng)多個方(fang)面，而吸收是**步。在(zai)細(xi)胞(bao)模型上(shang)進行內(nei)皮(pi)細(xi)胞(bao)通透性、藥物(wu)滲(shen)透等(deng)實(shi)驗(yan)(yan)中，默(mo)克生(sheng)命(ming)科學(xue)Millipore的(de)MillicellR細(xi)胞(bao)電(dian)(dian)(dian)(dian)阻儀(yi)和(he)細(xi)胞(bao)培(pei)養板是常(chang)用的(de)產(chan)品組合。MillicellRERS-2電(dian)(dian)(dian)(dian)阻儀(yi)是測(ce)(ce)(ce)定(ding)培(pei)養的(de)上(shang)皮(pi)細(xi)胞(bao)的(de)電(dian)(dian)(dian)(dian)阻或(huo)跨上(shang)皮(pi)細(xi)胞(bao)電(dian)(dian)(dian)(dian)阻(TEER)的(de)可靠設備。采用交流電(dian)(dian)(dian)(dian)(AC)定(ding)量檢測(ce)(ce)(ce)單層的(de)健康(kang)狀(zhuang)態和(he)細(xi)胞(bao)融合度(du)。兩個電(dian)(dian)(dian)(dian)極(ji)比較好的(de)Ag/AgCl用來測(ce)(ce)(ce)量電(dian)(dian)(dian)(dian)壓。由于電(dian)(dian)(dian)(dian)極(ji)設計得非常(chang)小(xiao)，實(shi)驗(yan)(yan)者可以非常(chang)方(fang)便地(di)測(ce)(ce)(ce)量微孔(kong)膜上(shang)生(sheng)長的(de)上(shang)皮(pi)細(xi)胞(bao)的(de)膜電(dian)(dian)(dian)(dian)壓及電(dian)(dian)(dian)(dian)阻。交流電(dian)(dian)(dian)(dian)設計與傳統直流電(dian)(dian)(dian)(dian)裝置相比也(ye)具有(you)一些優勢:膜電(dian)(dian)(dian)(dian)壓和(he)電(dian)(dian)(dian)(dian)極(ji)偏(pian)移(yi)不會影響電(dian)(dian)(dian)(dian)阻的(de)精確青浦(pu)區用于藥物(wu)ADME包括吸收分配(pei)等(deng)方(fang)面研究電(dian)(dian)(dian)(dian)阻儀(yi)