打(da)掃”。其(qi)他(ta)異(yi)物電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)破損(sun)更換電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)。電(dian)(dian)(dian)(dian)(dian)壓讀數儀(yi)表(biao)已連接到斷開儀(yi)表(biao)與充(chong)電(dian)(dian)(dian)(dian)(dian)器(qi)的(de)連接。高于20 mV充(chong)電(dian)(dian)(dian)(dian)(dian)器(qi)在“電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)電(dian)(dian)(dian)(dian)(dian)池(chi)電(dian)(dian)(dian)(dian)(dian)量低(di)給電(dian)(dian)(dian)(dian)(dian)池(chi)充(chong)電(dian)(dian)(dian)(dian)(dian)12小(xiao)時。|功能(neng)性|查看”電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)不(bu)平衡(heng)如表(biao)1所述調節電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)“使電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)平衡(heng)”。電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)頭(tou)變臟按照“電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)”中的(de)說明清潔電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)打(da)掃”。導(dao)電(dian)(dian)(dian)(dian)(dian)污染|檢(jian)查電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)間(jian)表(biao)面(mian)積電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)之間(jian)用于可能(neng)形成(cheng)導(dao)電(dian)(dian)(dian)(dian)(dian)的(de)材(cai)料內外之間(jian)的(de)橋(qiao)梁電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)。如果無(wu)法清理去除(chu)材(cai)料，更換電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)。電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)腐(fu)蝕檢(jian)查嵌入式銅電(dian)(dian)(dian)(dian)(dian)路走線導(dao)電(dian)(dian)(dian)(dian)(dian)跡線從電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)頭(tou)向(xiang)上(shang)延伸到處理。如果發(fa)(fa)現黑色(se)(se)變色(se)(se)在比較好，更換電(dian)(dian)(dian)(dian)(dian)極(ji)(ji)。 Millicell @ ERS-2儀(yi)表(biao)膜(mo)電(dian)(dian)(dian)(dian)(dian)壓范(fan)圍(wei)土200.0 mV電(dian)(dian)(dian)(dian)(dian)壓量測0.1毫伏電(dian)(dian)(dian)(dian)(dian)阻(zu)范(fan)圍(wei)0至(zhi)9上(shang)海益啟默克細胞(bao)跨膜(mo)電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)批發(fa)(fa)價(jia)。奉賢(xian)區(qu)Merck電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)咨詢報價(jia)

放(fang)電(dian)(dian)。為防止電(dian)(dian)池(chi)自身(shen)損壞 放(fang)電(dian)(dian)，然后每(mei)月為電(dian)(dian)池(chi)組(zu)充(chong)電(dian)(dian)。 更(geng)換電(dian)(dian)池(chi)： 1.使用1號梅花槽(cao)螺(luo)絲刀，從(cong)MillicellD上卸下(xia)(xia)四顆螺(luo)釘(ding)。 ERS-2外殼(ke)，儀器(qi)(qi)(qi)蓋(gai)與底座分開。 2.電(dian)(dian)池(chi)組(zu)通過(guo)金屬蓋(gai)安裝在(zai)基(ji)座上。 擰下(xia)(xia)兩顆螺(luo)絲，然后提起電(dian)(dian)池(chi)蓋(gai)和(he)(he)電(dian)(dian)池(chi)。 3.從(cong)蓋(gai)上取下(xia)(xia)電(dian)(dian)池(chi)組(zu)。 4.注意電(dian)(dian)池(chi)組(zu)連(lian)(lian)接(jie)(jie)器(qi)(qi)(qi)的(de)位置和(he)(he)電(dian)(dian)線顏(yan)色代碼(ma)，因此 新(xin)電(dian)(dian)池(chi)組(zu)可(ke)以保持正確的(de)極(ji)性。 拉動電(dian)(dian)路板(ban)(ban)上的(de)小塑料連(lian)(lian)接(jie)(jie)器(qi)(qi)(qi)，將(jiang)其(qi)斷(duan)開 從(cong)電(dian)(dian)路板(ban)(ban)上直接(jie)(jie)拉出來。 注意：以相反極(ji)性連(lian)(lian)接(jie)(jie)電(dian)(dian)池(chi)連(lian)(lian)接(jie)(jie)器(qi)(qi)(qi) 可(ke)能會導致人身(shen)傷害或設(she)備損壞。 5.將(jiang)新(xin)的(de)電(dian)(dian)池(chi)組(zu)放(fang)入電(dian)(dian)池(chi)蓋(gai)中(zhong)，然后重新(xin)固(gu)定(ding)到基(ji)座上。6.要(yao)將(jiang)電(dian)(dian)池(chi)組(zu)浦東(dong)新(xin)區在(zai)細(xi)胞模(mo)型上進行(xing)內皮細(xi)胞通透性實驗(yan)電(dian)(dian)阻儀聯系(xi)方(fang)式默克(ke)細(xi)胞跨膜電(dian)(dian)阻儀哪(na)家(jia)正規(gui)可(ke)靠(kao)？

紫外線分解銀/氯化銀電(dian)(dian)(dian)(dian)極(ji)。使(shi)(shi)用(yong)酒精或(huo)5％次(ci)氯酸鈉消(xiao)毒(du)/消(xiao)毒(du)，請遵循此步(bu)驟在層(ceng)流罩中(zhong)進行(xing)操作。1.將電(dian)(dian)(dian)(dian)極(ji)頭浸入消(xiao)毒(du)/消(xiao)毒(du)溶液中(zhong)15分鐘。風干15秒。注(zhu)意：請勿將電(dian)(dian)(dian)(dian)極(ji)頭放在酒精中(zhong)超過(guo)30分鐘一次(ci)連續浸泡會削弱電(dian)(dian)(dian)(dian)極(ji)的(de)(de)防護涂層(ceng)并縮短其(qi)使(shi)(shi)用(yong)壽命。2.在類似于電(dian)(dian)(dian)(dian)池的(de)(de)無(wu)菌(jun)電(dian)(dian)(dian)(dian)解質溶液中(zhong)沖洗電(dian)(dian)(dian)(dian)極(ji)培(pei)養(yang)(yang)基或(huo)o.1-0.15 M KCl或(huo)NaCl中(zhong)。3.為了(le)進行(xing)電(dian)(dian)(dian)(dian)阻測(ce)量(liang)，現在可以使(shi)(shi)用(yong)電(dian)(dian)(dian)(dian)極(ji)了(le)。4.對于電(dian)(dian)(dian)(dian)壓測(ce)量(liang)，允許電(dian)(dian)(dian)(dian)極(ji)在無(wu)菌(jun)狀態下(xia)達到平衡類似于細胞培(pei)養(yang)(yang)基的(de)(de)電(dian)(dian)(dian)(dian)解質溶液處理15分鐘。測(ce)量(liang)電(dian)(dian)(dian)(dian)池電(dian)(dian)(dian)(dian)阻MillicellR ERS-2系統的(de)(de)優先(xian)選(xuan)擇應用(yong)是測(ce)量(liang)電(dian)(dian)(dian)(dian)池中(zhong)的(de)(de)電(dian)(dian)(dian)(dian)阻。要測(ce)

或者(zhe)殺菌(jun)劑滅菌(jun)。不能(neng)使用(yong)高(gao)溫或者(zhe)火焰(yan)滅菌(jun)，因為(wei)電(dian)(dian)(dian)極(ji)(ji)無(wu)法承受高(gao)溫。乙醇滅菌(jun)流程如(ru)下:1.將電(dian)(dian)(dian)極(ji)(ji)插入70%乙醇浸泡(pao)15min進(jin)(jin)行滅菌(jun)，取(qu)出(chu)電(dian)(dian)(dian)極(ji)(ji)風干15秒;注(zhu)意:不能(neng)將電(dian)(dian)(dian)極(ji)(ji)浸入乙醇中超過30min，這會損壞電(dian)(dian)(dian)極(ji)(ji)的保護層，縮短其使用(yong)壽命。2.用(yong)與(yu)培養(yang)基(ji)相似的電(dian)(dian)(dian)極(ji)(ji)液（或者(zhe)0.1-0.15M NaCVKCI沖洗電(dian)(dian)(dian)極(ji)(ji);3.若是(shi)進(jin)(jin)行電(dian)(dian)(dian)阻(zu)測量(liang)(liang)(liang)，此時電(dian)(dian)(dian)極(ji)(ji)已可(ke)(ke)以(yi)使用(yong);4.若是(shi)進(jin)(jin)行電(dian)(dian)(dian)壓測量(liang)(liang)(liang)，將電(dian)(dian)(dian)極(ji)(ji)在(zai)與(yu)培養(yang)基(ji)相似的無(wu)菌(jun)電(dian)(dian)(dian)極(ji)(ji)緩(huan)沖液中平衡15分鐘。注(zhu)意:為(wei)了保持無(wu)菌(jun)，可(ke)(ke)以(yi)將電(dian)(dian)(dian)極(ji)(ji)放在(zai)紫(zi)外燈下短時間照射。三(san)．電(dian)(dian)(dian)阻(zu)測量(liang)(liang)(liang)（量(liang)(liang)(liang)程:0-9999o，靈敏度:1Q)注(zhu)意:雖然儀表上(shang)(shang)海益啟細(xi)胞跨膜電(dian)(dian)(dian)阻(zu)儀用(yong)于跨上(shang)(shang)皮電(dian)(dian)(dian)阻(zu)的測量(liang)(liang)(liang)。

行(xing)(xing)電極平衡4.未培(pei)(pei)養(yang)(yang)細(xi)胞(bao)(bao)(bao)的(de)(de)Millicell培(pei)(pei)養(yang)(yang)皿(min)(對照)5.培(pei)(pei)養(yang)(yang)了細(xi)胞(bao)(bao)(bao)的(de)(de)Millicell培(pei)(pei)養(yang)(yang)皿(min)6.70%的(de)(de)乙醇7.3/32"平頭螺絲刀MillicellR細(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)板或(huo)(huo)細(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)小室(shi):PET膜(mo)(mo)或(huo)(huo)PCF膜(mo)(mo)，膜(mo)(mo)孔(kong)(kong)(kong)徑為0.4um或(huo)(huo)1um常用(yong)的(de)(de)產(chan)品包括:·目(mu)錄(lu)號PSHTO10R5: PCF膜(mo)(mo)，膜(mo)(mo)孔(kong)(kong)(kong)徑0.4um 24孔(kong)(kong)(kong)板-目(mu)錄(lu)號PSRPO04R5: PET膜(mo)(mo)，膜(mo)(mo)孔(kong)(kong)(kong)徑1.oum,96孔(kong)(kong)(kong)膜(mo)(mo)板-目(mu)錄(lu)號PSHTO04R5: PCF膜(mo)(mo)，膜(mo)(mo)孔(kong)(kong)(kong)徑0.4um,96孔(kong)(kong)(kong)膜(mo)(mo)板-目(mu)錄(lu)號MACACORS5: Caco2無菌帶蓋96孔(kong)(kong)(kong)板。 Caco-2細(xi)胞(bao)(bao)(bao)在細(xi)胞(bao)(bao)(bao)小室(shi)上培(pei)(pei)養(yang)(yang)0-90％非冷凝相對濕度方面7.25x 4.25x 2.3英寸(cun)（18.4x 10.8 x 5.8厘米）上海益啟默克細(xi)胞(bao)(bao)(bao)跨膜(mo)(mo)電阻儀的(de)(de)供應(ying)商。普陀區(qu)在細(xi)胞(bao)(bao)(bao)模型上進行(xing)(xing)內(nei)皮細(xi)胞(bao)(bao)(bao)通透性實驗電阻儀批發

上海益(yi)啟細胞跨膜電阻儀用于藥物ADME包(bao)括(kuo)吸(xi)收(shou)，分(fen)配(pei)、代謝(xie)、排泄和毒性(xing)等方面研究。奉賢區Merck電阻儀電阻儀咨(zi)詢報(bao)價(jia)

P03050Millicell?-ERS-2電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)是(shi)測量(liang)電(dian)(dian)(dian)(dian)(dian)壓(ya)和電(dian)(dian)(dian)(dian)(dian)阻(zu)的(de)可(ke)靠設備，常(chang)被用(yong)(yong)來(lai)(lai)測定(ding)培養的(de)上皮(pi)細(xi)胞(bao)(bao)的(de)電(dian)(dian)(dian)(dian)(dian)阻(zu)或跨(kua)上皮(pi)細(xi)胞(bao)(bao)電(dian)(dian)(dian)(dian)(dian)阻(zu)(TEER)。ERS-2采用(yong)(yong)交(jiao)流(liu)電(dian)(dian)(dian)(dian)(dian)(AC)定(ding)量(liang)檢測單層(ceng)的(de)健康狀態和細(xi)胞(bao)(bao)融合度。 兩個電(dian)(dian)(dian)(dian)(dian)極的(de)Ag/AgCl用(yong)(yong)來(lai)(lai)測量(liang)電(dian)(dian)(dian)(dian)(dian)壓(ya)。由于電(dian)(dian)(dian)(dian)(dian)極設計得非常(chang)小(xiao)，實驗者可(ke)以非常(chang)方便地測量(liang)微(wei)孔膜上生(sheng)長的(de)上皮(pi)細(xi)胞(bao)(bao)的(de)膜電(dian)(dian)(dian)(dian)(dian)壓(ya)及電(dian)(dian)(dian)(dian)(dian)阻(zu)。交(jiao)流(liu)電(dian)(dian)(dian)(dian)(dian)設計與傳統直(zhi)流(liu)電(dian)(dian)(dian)(dian)(dian)裝置相比也具(ju)有一些(xie)優勢：膜電(dian)(dian)(dian)(dian)(dian)壓(ya)和電(dian)(dian)(dian)(dian)(dian)極偏(pian)移(yi)不會影(ying)響電(dian)(dian)(dian)(dian)(dian)阻(zu)的(de)精確測定(ding)；細(xi)胞(bao)(bao)上的(de)零靜電(dian)(dian)(dian)(dian)(dian)荷消除(chu)了DC電(dian)(dian)(dian)(dian)(dian)流(liu)對細(xi)胞(bao)(bao)膜產生(sheng)的(de)不利(li)影(ying)響；沒有金屬電(dian)(dian)(dian)(dian)(dian)極的(de)電(dian)(dian)(dian)(dian)(dian)化學沉積問(wen)題。 藥物研究ADME包括吸收、分配、代謝、排奉賢區(qu)Merck電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)電(dian)(dian)(dian)(dian)(dian)阻(zu)儀(yi)咨詢報價